Enzymes and Inhibitors
Thermo Scientific™ FastDigest Bsp119I
Cut at TT^CGAA sites with Thermo Scientific™ FastDigest™ Bsp119I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest FspBI
Cut at C^TAG sites with Thermo Scientific™ FastDigest™ BfaI (FspBI), which performs best using universal FastDigest Buffer (Isoschizomers: BfaI, MaeI, XspI).
Thermo Scientific™ Cfr10I Buffer (10X)
Optimize digestions with Cfr10I restriction enzyme using this buffer, premixed with BSA for enhanced stability.
β-Nicotinamide Adenine Dinucleotide, Oxidized Form, MP Biomedicals™
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
Thermo Scientific™ EheI (SfoI) (10 U/μL)
Cut at GGC^GCC sites with EheI (SfoI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).
MP Biomedicals™ Glucose-6-phosphate Dehydrogenase
Glucose-6-phosphate Dehydrogenase is an enzyme in pentose phosphate pathway.
Thermo Scientific™ T4 beta-glucosyltransferase
Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis
Thermo Scientific™ Cfr10I (BsrFI) (10 U/μL)
Cut R^CCGGY sites with Cfr10I (BsrFI) restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: Bse118I, BsrFI, BssAIm).
Thermo Scientific™ LweI (SfaNI) (10 U/μL)
The LweI (SfaNI) restriction enzyme recognizes GCATC(5/9)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: SfaNI).
Invitrogen™ T4 DNA Ligase (5 U/μL)
Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini
MP Biomedicals™ Pyruvate Kinase from Rabbit Muscle
≥200u/mg protein, White Crystalline Powder
Thermo Scientific™ FastDigest Alw26I (IIs class)
Cut at GTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ Alw26I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest TaiI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.